The word incubation (from the Latin incubare, "to lie upon") may refer to:
- Avian incubation, sitting on or brooding bird's eggs in order to hatch them
- Incubation period, a medical term for the time between being exposed to infection and showing first symptoms
- Business incubator, for startup businesses
- Incubation (psychology), the process of thinking about a problem subconsciously while being involved in other activities
- Incubation (ritual), the religious practice of sleeping in a sacred area with the intention of experiencing a divinely-inspired dream or cure
- Incubator (microbiology), a process for maintaining a bacterial culture at a particular temperature for a set length of time, in order to measure bacterial growth
- Incubation: Time Is Running Out, a 1997 computer game
- "Incubator", a 2001 episode of the American TV series Farscape
- Incubation for newborn infants, such as in a Neonatal intensive care unit
- "Incubation", a song released on the B-side of the Komakino flexi-single in 1980, and on the Substance (Joy Division album) in 1988
- Software incubation: software and community verification phase as done in the Apache Software Foundation and OSGeo Foundation
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